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KMID : 0603520010060020076
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Journal of Korean Association of Cancer Prevention
2001 Volume.6 No. 2 p.76 ~ p.84
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Inhibition Effects of Galangin against 2-AAF Induced in vitro and in vivo Unscheduled DNA Synthesis
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Kim Jong-Won
Han Eui-Sik
Eom Mi-Ok
Park Mi-Sun
Lee Jin-Joo
Jung Hai-Kwan
Oh Hye-Young
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Abstract
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Unscheduled DNA synthesis (UDS) test is well validated and known as one of genetic toxicity tests. UDS provides information as an indicative of DNA synthesis and DNA repair. The purpose of this study is that we would like to help for many researchers to evaluate test substances (drug, food additive and so on) correctly and its application to assess antimutagenic effect. For this purpose, according to SOP, we have performed in vitro and in vivo UDS test in male rat hepatocyte treated with 2-acetylaminofluorene (2-AAF) as a positive control compound. Thereafter, we have selected galangin which is one of anti-oxidants and flavonoids, and performed UDS test about galangin. And we have studied antimutagenic effect of galangin against 2-AAF using in vitro and in vivo UDS test in male rat hepatocyte. In the in vitro UDS test, 2-AAF showed significantly increase UDS from 10-8M to 10-5M. According to above results, we selected 10-6M 2-AAF as a co-treatment concentration which was shown highest increase UDS. The percentage of UDS in treated all concentration of galangin was similar to that of negative control. When the galangin co-treated with 2-AAF, galangin inhibited 2-AAF-induced UDS up to 80%. In the in vivo UDS test, 2-AAF showed significantly increase UDS in 0.75, 7.5, 75 ㎎/㎏. According to above results, we selected 75 ㎎/㎏ 2-AAF as a co-treatment concentration which was shown highest increase UDS. The galangin didn’t show any increase compared with negative control in all doses. When the galangin co-treated with 2-AAF, galangin inhibited 2-AAF-induced UDS up to 83%.
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KEYWORD
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in vitro unscheduled DNA synthesis, in vivo unscheduled DNA synthesis, 2-acetylaminofluorene, Galangin
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